LOCUS       40924_35763       12416 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPZP 35S attR-hRluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Subramanian C, Woo J, Cai X, Xu X, Servick S, Johnson CH, Nebenfuhr 
            A, von Arnim AG.
  TITLE     A suite of tools and application notes for in vivo protein 
            interaction assays using bioluminescence resonance energy transfer 
            (BRET)
  JOURNAL   Plant J. 48 (1), 138-152 (2006)
  PUBMED    16925598
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   von Arnim AG, Subramanian C.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2005) BCMB, University of Tennessee Knoxville, 
            1406 Circle Drive, Hesler Biology Bldg 618, Knoxville, TN 37996, USA
REFERENCE   3  (bases 1 to 12416)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2006"; volume: "48"; issue: "1"; pages: "138-152"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2005) BCMB, University of Tennessee Knoxville, 1406 Circle 
            Drive, Hesler Biology Bldg 618, Knoxville, TN 37996, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1813
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2250..3314
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4061
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4247..4835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5082..5870)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    6398..6422
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6520..6694)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     6909..6925
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7030..7701
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    7849..7973
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        7998..8028
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             8082..8738
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             9083..9385
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(9429..9553)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             9555..10487
                     /label=hRluc
                     /note="Renilla luciferase"
     regulatory      10500..10710
                     /label=35S cauliflower mosaic virus terminator
                     /note="35S cauliflower mosaic virus terminator"
                     /regulatory_class="terminator"
     promoter        10773..11153
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             11159..11707
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      11720..11972
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(12000..12016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12024..12040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12048..12078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12093..12114)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    12279..12303
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
     4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
     5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
     6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
     6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6481 acgccgaatt aattcggggg atctataggg actttaggtg atctggattt tagtactgga
     6541 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
     6601 ctaagggttt cttatatgct caacacgtga gcgaaaccct ataagaaccc taattccctt
     6661 atctgggaac tactcacaca ttattatgga gaaactcgat gtcgatcgac tctagctaga
     6721 acgaattgtt aggtggcggt acttgggtcg atttggcaag ctgctctagc attcgccatt
     6781 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     6841 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     6901 acgacgttgt aaaacgacgg ccagtgccaa gcttgatatc gaattcctgc aggtcaacat
     6961 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     7021 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     7081 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     7141 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     7201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     7261 aaagcaagtg gattgatgtg ataacatggt ggagcacgac acacttgtct actccaaaaa
     7321 tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac aaagggtaat
     7381 atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg tgaagatagt
     7441 ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga
     7501 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     7561 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     7621 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     7681 ttcatttcat ttggagagga cctcgagaat tctcaacaca acatatacaa aacaaacgaa
     7741 tctcaagcaa tcaagcattc tacttctatt gcagcaattt aaatcatttc ttttaaagca
     7801 aaagcaattt tctgaaaatt ttcaccattt acgaacgata ggcctaggac aagtttgtac
     7861 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     7921 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
     7981 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     8041 gttaggatcc ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     8101 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     8161 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     8221 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     8281 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     8341 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     8401 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     8461 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     8521 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     8581 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     8641 tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg
     8701 aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac
     8761 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     8821 ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt
     8881 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     8941 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     9001 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     9061 gacgagaaca gggactggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     9121 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     9181 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     9241 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     9301 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     9361 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     9421 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     9481 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     9541 tgtacaaagt ggtcatggct tcgaaggtgt acgaccccga gcagaggaag aggatgatca
     9601 ccggccccca gtggtgggcc aggtgcaagc agatgaacgt gctggacagc ttcatcaact
     9661 actacgacag cgagaagcac gccgagaacg ccgtgatctt cctgcacggc aacgccgcta
     9721 gcagctacct gtggaggcac gtggtgcccc acatcgagcc cgtggccagg tgcatcatcc
     9781 ccgatctgat cggcatgggc aagagcggca agagcggcaa cggcagctac aggctgctgg
     9841 accactacaa gtacctgacc gcctggttcg agctcctgaa cctgcccaag aagatcatct
     9901 tcgtgggcca cgactggggc gcctgcctgg ccttccacta cagctacgag caccaggaca
     9961 agatcaaggc catcgtgcac gccgagagcg tggtggacgt gatcgagagc tgggacgagt
    10021 ggccagacat cgaggaggac atcgccctga tcaagagcga ggagggcgag aagatggtgc
    10081 tggagaacaa cttcttcgtg gagaccatgc tgcccagcaa gatcatgaga aagctggagc
    10141 ccgaggagtt cgccgcctac ctggagccct tcaaggagaa gggcgaggtg agaagaccca
    10201 ccctgagctg gcccagagag atccccctgg tgaagggcgg caagcccgac gtggtgcaga
    10261 tcgtgagaaa ctacaacgcc tacctgagag ccagcgacga cctgcccaag atgttcatcg
    10321 agagcgaccc cggcttcttc agcaacgcca tcgtggaggg cgccaagaag ttccccaaca
    10381 ccgagttcgt gaaggtgaag ggcctgcact tcagccagga ggacgccccc gacgagatgg
    10441 gcaagtacat caagagcttc gtggagagag tgctgaagaa cgagcagaga tctatctaga
    10501 gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat
    10561 aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt
    10621 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
    10681 tttctaattc ctaaaaccaa aatccagtga cctgcagccc gggggatcca ctagagcggc
    10741 cgccaccggg taccgagctc gaattctgag atttttcaaa tcagtgcgca agacgtgacg
    10801 taagtatctg agctagtttt tatttttcta ctaatttggt cgtttatttc ggcgtgtagg
    10861 acatggcaac cgggcctgaa tttcgcgggt attctgtttc tattccaact ttttcttgat
    10921 ccgcagccat taacgacttt tgaatagata cgctgacacg ccaagcctcg ctagtcaaaa
    10981 gtgtaccaaa caacgcttta cagcaagaac ggaatgcgcg tgacgctcgc ggtgacgcca
    11041 tttcgccttt tcagaaatgg ataaatagcc ttgcttccta ttatatcttc ccaaattacc
    11101 aatacattac actagcatct gaatttcata accaatctcg atacaccaaa tcgaatcgat
    11161 gagcccagaa cgacgcccgg ccgacatccg ccgtgccacc gaggcggaca tgccggcggt
    11221 ctgcaccatc gtcaaccact acatcgagac aagcacggtc aacttccgta ccgagccgca
    11281 ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg gagcgctatc cctggctcgt
    11341 cgccgaggtg gacggcgagg tcgccggcat cgcctacgcg ggcccctgga aggcacgcaa
    11401 cgcctacgac tggacggccg agtcgaccgt gtacgtctcc ccccgccacc agcggacggg
    11461 actgggctcc acgctctaca cccacctgct gaagtccctg gaggcacagg gcttcaagag
    11521 cgtggtcgct gtcatcgggc tgcccaacga cccgagcgtg cgcatgcacg aggcgctcgg
    11581 atatgccccc cgcggcatgc tgcgggcggc cggcttcaag cacgggaact ggcatgacgt
    11641 gggtttctgg cagctggact tcagcctgcc ggtaccgccc cgtccggtcc tgcccgtcac
    11701 cgagatctaa ctgcagaatc ttggactccc atgttggcaa aggcaaccaa acaaacaatg
    11761 aatgatccgc tcctgcatat ggggcggttt gagtatttca actgccattt gggctgaatt
    11821 gaagacatgc tcctgtcaga aattccgtga tcttactcaa tattcagtaa tctcggccaa
    11881 tatcctaaat gtgcgtggct ttatctgtct ttgtattgtt tcatcaattc atgtaacgtt
    11941 tgcttttctt atgaattttc aaataaatta tcagatccga gctcgaattc gtaatcatgg
    12001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    12061 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    12121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    12181 ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgag cttggatcag
    12241 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
    12301 aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt
    12361 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaa
//
